Abstract: The unique geographical environment and high altitude span of Guizhou Province provide abundant honey source plant resources for the production of Rhus chinensis Mill. honey. The mineral content in honey varies with altitude and other factors. This study collected gallnut honey from 10 different altitude farms in Guizhou Province, and determined the content of magnesium, manganese, iron, copper, zinc, sodium, potassium, calcium, phosphorus, and selenium in it. The results indicate that the total mineral content ranges from 381.93 to 882.81 mg/kg. The sodium content ranges from 12.43 to 20.98 mg/kg. Potassium content range 141.17 to 540.33mg/kg. Potassium sodium ratio is 7.94 to 38.05. Selenium was not detected in any of the 10 samples of gallnut honey. Establishing this database is beneficial for the quality evaluation of local honey and providing relevant data for the development of characteristic honey.